SEQUENCE AND STRUCTURE OF THE CATALYTIC RNA OF HEPATITIS DELTA-VIRUS GENOMIC RNA

被引:76
作者
WU, HN
WANG, YJ
HUNG, CF
LEE, HJ
LAI, MMC
机构
[1] UNIV SO CALIF, SCH MED, HOWARD HUGHES MED INST, LOS ANGELES, CA 90033 USA
[2] UNIV SO CALIF, SCH MED, DEPT MICROBIOL, LOS ANGELES, CA 90033 USA
关键词
HEPATITIS DELTA-VIRUS RNA; RIBOZYME; SECONDARY STRUCTURE; TRANS-CLEAVAGE; SITE-SPECIFIC MUTAGENESIS;
D O I
10.1016/0022-2836(92)90728-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human hepatitis delta virus (HDV) RNA has been shown to contain a self-catalyzed cleavage activity. The sequence requirement for its catalytic activity appears to be different from that of other known ribozymes. In this paper, we define the minimum contiguous sequence and secondary structure of the HDV genomic RNA required for the catalytic activity. By using nested-set deletion mutants, we have determined that the essential sequence for the catalytic activity is contained within no more than 85 nucleotides of HDV RNA. These results are in close agreement with the previous determinations and confirmed the relative insignificance of the sequence at the 5′ side of the cleavage site. The smallest catalytic RNA, representing HDV genomic RNA nucleotide positions 683 to 770, was used as the basis for studying the secondary structure requirements for catalytic activity. Analysis of the RNA structure, using RNase V1, nuclease S1 and diethylpyrocarbonate treatments showed that this RNA contains at least two stem-and-loop structures. Other larger HDV RNA subfragments containing the catalytic activity also have a very similar secondary structure. By performing site-specific mutagenesis studies, it was shown that one of the stem-and-loop structures could be deleted to half of its original size without affecting the catalytic activity. In addition, the other stem-and-loop contained a six base-pair helix, and the structure, rather than the sequence, of this helix was required for the catalytic activity. However, the structure of a portion of the stem-and-loop remains uncertain. We also report that this RNA can be divided into two separate molecules, which alone did not have cleavage activity but, when mixed, one of the RNAs could be cleaved in trans. This study thus reveals some features of the secondary structure of the HDV genomic RNA involved in self-catalyzed cleavage. A model of this RNA structure is presented. © 1992.
引用
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页码:233 / 245
页数:13
相关论文
共 19 条
[1]   TAT TRANS-ACTIVATES THE HUMAN IMMUNODEFICIENCY VIRUS THROUGH A NASCENT RNA TARGET [J].
BERKHOUT, B ;
SILVERMAN, RH ;
JEANG, KT .
CELL, 1989, 59 (02) :273-282
[2]   MOLECULAR-CLONING AND CHARACTERIZATION OF AN ISOLATE OF HEPATITIS DELTA VIRUS FROM TAIWAN [J].
CHAO, YC ;
LEE, CM ;
TANG, HS ;
GOVINDARAJAN, S ;
LAI, MMC .
HEPATOLOGY, 1991, 13 (02) :345-352
[3]  
CHAO YC, 1990, VIROLOGY, V178, P384
[4]   SELF-CLEAVING TRANSCRIPTS OF SATELLITE DNA FROM THE NEWT [J].
EPSTEIN, LM ;
GALL, JG .
CELL, 1987, 48 (03) :535-543
[5]   2 SEQUENCES PARTICIPATING IN THE AUTOLYTIC PROCESSING OF SATELLITE TOBACCO RINGSPOT VIRUS COMPLEMENTARY RNA [J].
FELDSTEIN, PA ;
BUZAYAN, JM ;
BRUENING, G .
GENE, 1989, 82 (01) :53-61
[6]   SELF-CLEAVAGE OF PLUS AND MINUS RNAS OF A VIRUSOID AND A STRUCTURAL MODEL FOR THE ACTIVE-SITES [J].
FORSTER, AC ;
SYMONS, RH .
CELL, 1987, 49 (02) :211-220
[7]   HAIRPIN CATALYTIC RNA MODEL - EVIDENCE FOR HELICES AND SEQUENCE REQUIREMENT FOR SUBSTRATE RNA [J].
HAMPEL, A ;
TRITZ, R ;
HICKS, M ;
CRUZ, P .
NUCLEIC ACIDS RESEARCH, 1990, 18 (02) :299-304
[8]   RNA CATALYTIC PROPERTIES OF THE MINIMUM (-)STRSV SEQUENCE [J].
HAMPEL, A ;
TRITZ, R .
BIOCHEMISTRY, 1989, 28 (12) :4929-4933
[9]   MOLECULAR-CLONING AND SEQUENCING OF A HUMAN HEPATITIS-DELTA (DELTA) VIRUS-RNA [J].
MAKINO, S ;
CHANG, MF ;
SHIEH, CK ;
KAMAHORA, T ;
VANNIER, DM ;
GOVINDARAJAN, S ;
LAI, MMC .
NATURE, 1987, 329 (6137) :343-346
[10]  
Maniatis T., 1982, MOL CLONING