THE ARSR PROTEIN IS A TRANS-ACTING REGULATORY PROTEIN

被引：154

作者：

WU, J ^{[1
]}

ROSEN, BP ^{[1
]}

机构：

[1] WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM,DETROIT,MI 48201

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00779.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The arsR gene encodes the regulatory protein of the plasmid-encoded arsenical resistance operon. A series of in-frame fusions was constructed between the C-terminally truncated arsR gene and the coding region for the mature form of beta-lactamase (blaM). Fusions containing most of the arsR gene were still inducible by arsenicals. Fusions containing less than 102 residues of the 117-residue ArsR protein were constitutive. When a wild-type arsR gene was placed in trans, the constitutive constructs were again inducible. The results demonstrate that the ArsR protein is a trans-acting regulatory protein which controls its own expression.

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页码：1331 / 1336

页数：6

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