DIFFERENCES IN THE PROBABILITY OF CLONING SPECIFIC DNA BETWEEN PRIMARY AND AMPLIFIED LIBRARIES - THEORETICAL CONSIDERATIONS

被引：2

作者：

KUBO, A

KONDO, N

机构：

[1] National Institute for Environmental Studies, Tsukuba, Ibaraki

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1991年 / 8卷 / 08期

关键词：

D O I：

10.1016/1050-3862(91)90034-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a theoretical study of the probability of isolating a particular clone from a DNA library. There are differences in this probability between primary and amplified libraries even if the desired clone represents the same fraction of both libraries. As the result, we must screen severalfold more phage or bacteria in an amplified library than in a primary library.

引用

页码：238 / 239

页数：2