ALTERATION OF SUPERHELICAL STATE OF DNA BY ALUMINUM (AL)

被引：25

作者：

RAO, KSJ ^{[1
]}

RAO, BS ^{[1
]}

VISHNUVARDHAN, D ^{[1
]}

PRASAD, KVS ^{[1
]}

机构：

[1] CENT FOOD TECHNOL RES INST,FOOD CHEM UNIT,MYSORE 570013,KARNATAKA,INDIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1172卷 / 1-2期

关键词：

ALUMINUM; SUPERCOILED DNA;

D O I：

10.1016/0167-4781(93)90263-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of aluminium (Al) on the supercoiled state of pUC18 DNA was studied by ethidium bromide fluorescence and agarose gel electrophoresis. Al at physiologically relevant concentrations relaxed the intact supercoiled DNA as well as the topoisomers induced by chloroquine. EDTA prevented the unwinding effect of Al on supercoiled DNA. Al did not alter the mobility of linear DNA in agarose gels. The implications of this finding in neurological disorders are discussed.

引用

页码：17 / 20

页数：4

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