OPTIMIZATION OF SEPARATION SELECTIVITY IN CAPILLARY ELECTROPHORESIS OF FLAVONOIDS

被引：28

作者：

PIETTA, PG ^{[1
]}

MAURI, PL ^{[1
]}

ZINI, L ^{[1
]}

GARDANA, C ^{[1
]}

机构：

[1] CNR,IST TECNOL BIOMED AVANZATE,I-20131 MILAN,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 680卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80065-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The migration behaviour of selected flavonoids differing in their degree of hydroxylation was investigated. The influence of the solvent in the injected sample, the surfactant [sodium dodecyl sulphate (SDS)] and pH on the separation selectivity was studied. The organic solvent (methanol or 2-propanol) modifies the interaction between micelles and analytes, thus reducing migration times and resolution. SDS improves the separation at pH 8.3, whereas it has less or no effect at higher pH. At pH 10.5 the separation is mainly regulated by ionization of the hydroxyl groups and berate complexation of the carbohydrate residues.

引用

页码：175 / 179

页数：5

共 8 条

[1] CAFFEOYLQUINIC ACIDS FROM FLOWERS OF ARNICA-MONTANA AND ARNICA-CHAMISSONIS [J].