ROLE OF YOPH IN THE SUPPRESSION OF TYROSINE PHOSPHORYLATION AND RESPIRATORY BURST ACTIVITY IN MURINE MACROPHAGES INFECTED WITH YERSINIA-ENTEROCOLITICA

Tyrosine phosphorylation is an important component of the signaling pathways responsible for the activation of the macrophage respiratory burst, Because the virulence plasmid of Yersinia enterocolitica encodes a phosphotyrosine phosphatase, YopH, it is possible that the pathogenic strategy of Y. enterocolitica involves the disruption of tyrosine phosphorylation in the macrophage leading to inhibition of respiratory burst activity. We have investigated the effects of Yersinia infection on tyrosine phosphorylation and respiratory burst activity in murine bone marrow-derived macrophages, Infection of macrophages with virulent [Ye(pYV(+))] but not avirulent [Ye(pYV(-))] strains of Y. enterocolitica was found to suppress both tyrosine phosphorylation and respiratory burst activity in response to zymosan, Mutational inactivation of YopH reversed the suppressive effect of Ye(pYV(+)) on zymosan-induced tyrosine phosphorylation, indicating that YopH is responsible for the dephosphorylation of macrophage phosphotyrosine-containing proteins observed in macrophages infected with the virulent strain of Y. enterocolitica. In contrast, mutational loss of YopH failed to reverse the inhibitory effect of Ye(pYV(+)) on the zymosan-triggered respiratory burst, We conclude that the inhibition of the macrophage respiratory burst by Y. enterocolitica involves a plasmid-encoded virulence protein(s) other than, or in addition to, YopH.