SENSITIVE CHEMILUMINESCENCE ENZYME-IMMUNOASSAY FOR VASCULAR ENDOTHELIAL GROWTH-FACTOR VASCULAR-PERMEABILITY FACTOR IN HUMAN SERUM

被引：52

作者：

HANATANI, M

TANAKA, Y

KONDO, S

OHMORI, I

SUZUKI, H

机构：

[1] Department of Bioscience Research, Tsukuba Research Laboratory, Toagosei Co., Ltd., Tsukuba, Ibaraki 300-33

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 10期

关键词：

D O I：

10.1271/bbb.59.1958

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sandwich chemiluminescence enzyme inmunoassay for measuring the level of VEGF/VPF in serum was constructed. The detectability of the assay is very Low (1.0 pg/ml) and the measurable range of the assay was very wide (1-1000 pg/ml). The assay showed that the average level of VEGF/VPF in human sera from healthy blood donors was approximately 19 pg/ml.

引用

页码：1958 / 1959

页数：2

共 17 条

[1] VASCULAR ENDOTHELIAL GROWTH-FACTOR IN OCULAR FLUID OF PATIENTS WITH DIABETIC-RETINOPATHY AND OTHER RETINAL DISORDERS
AIELLO, LP
AVERY, RL
ARRIGG, PG
KEYT, BA
JAMPEL, HD
SHAH, ST
PASQUALE, LR
THIEME, H
IWAMOTO, MA
PARK, JE
NGUYEN, HV
AIELLO, LM
FERRARA, N
KING, GL
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (22) : 1480 - 1487
[2] 1,2-DIOXETANES - NOVEL CHEMI-LUMINESCENT ENZYME SUBSTRATES - APPLICATIONS TO IMMUNOASSAYS
BRONSTEIN, I
EDWARDS, B
VOYTA, JC
[J]. JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1989, 4 (01): : 99 - 111
[3] TUMOR VASCULAR-PERMEABILITY FACTOR STIMULATES ENDOTHELIAL-CELL GROWTH AND ANGIOGENESIS
CONNOLLY, DT
HEUVELMAN, DM
NELSON, R
OLANDER, JV
EPPLEY, BL
DELFINO, JJ
SIEGEL, NR
LEIMGRUBER, RM
FEDER, J
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (05) : 1470 - 1478
[4] VASCULAR-PERMEABILITY FACTOR ENDOTHELIAL GROWTH-FACTOR (VPF/VEGF) - ACCUMULATION AND EXPRESSION IN HUMAN SYNOVIAL-FLUIDS AND RHEUMATOID SYNOVIAL TISSUE
FAVA, RA
OLSEN, NJ
SPENCERGREEN, G
YEO, KT
YEO, TK
BERSE, B
JACKMAN, RW
SENGER, DR
DVORAK, HF
BROWN, LF
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 180 (01) : 341 - 346
[5] PITUITARY FOLLICULAR CELLS SECRETE A NOVEL HEPARIN-BINDING GROWTH-FACTOR SPECIFIC FOR VASCULAR ENDOTHELIAL-CELLS
FERRARA, N
HENZEL, WJ
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (02) : 851 - 858
[6] WHAT IS THE EVIDENCE THAT TUMORS ARE ANGIOGENESIS DEPENDENT
FOLKMAN, J
[J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1990, 82 (01): : 4 - 6
[7] THE VASCULAR ENDOTHELIAL GROWTH-FACTOR FAMILY - IDENTIFICATION OF A 4TH MOLECULAR-SPECIES AND CHARACTERIZATION OF ALTERNATIVE SPLICING OF RNA
HOUCK, KA
FERRARA, N
WINER, J
CACHIANES, G
LI, B
LEUNG, DW
[J]. MOLECULAR ENDOCRINOLOGY, 1991, 5 (12) : 1806 - 1814
[8] HOUCK KA, 1992, J BIOL CHEM, V267, P26031
[9] ENZYME-LABELING OF ANTIBODIES AND THEIR FRAGMENTS FOR ENZYME-IMMUNOASSAY AND IMMUNOHISTOCHEMICAL STAINING
ISHIKAWA, E
IMAGAWA, M
HASHIDA, S
YOSHITAKE, S
HAMAGUCHI, Y
UENO, T
[J]. JOURNAL OF IMMUNOASSAY, 1983, 4 (03): : 209 - 327
[10] VASCULAR-PERMEABILITY FACTOR, AN ENDOTHELIAL-CELL MITOGEN RELATED TO PDGF
KECK, PJ
HAUSER, SD
KRIVI, G
SANZO, K
WARREN, T
FEDER, J
CONNOLLY, DT
[J]. SCIENCE, 1989, 246 (4935) : 1309 - 1312

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