REGULATION OF HUMAN-LEUKOCYTE P21-ACTIVATED KINASES THROUGH G-PROTEIN-COUPLED RECEPTORS

被引：360

作者：

KNAUS, UG

MORRIS, S

DONG, HJ

CHERNOFF, J

BOKOCH, GM

机构：

[1] Scripps Res Inst, DEPT CELL BIOL, LA JOLLA, CA 92037 USA

[2] FOX CHASE CANC CTR, PHILADELPHIA, PA 19111 USA

来源：

SCIENCE | 1995年 / 269卷 / 5221期

关键词：

D O I：

10.1126/science.7618083

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Rac guanosine 5'-triphosphate (GTP)-binding proteins regulate oxidant production by phagocytic leukocytes. Two Ste20-related p21-activated kinases (PAKs) were identified as targets of Rac in human neutrophils. Activity of the similar to 65- and similar to 68-kilodalton PAKs was rapidly stimulated by chemoattractants acting through pertussis toxin-sensitive heterotrimeric GTP-binding proteins (G proteins). Native and recombinant PAKs phos phorylated the p47(phox) reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase component in a Rac-GTP-dependent manner. The action of PAKs during phagocyte activation by G protein-coupled pathways may contribute to regulation of NADPH oxidase activity.

引用

页码：221 / 223

页数：3

共 31 条

[1] ACTIVATION OF THE NADPH OXIDASE INVOLVES THE SMALL GTP-BINDING PROTEIN P21RAC1 [J].