THE DESIGN OF A SIMPLE COMPETITIVE ELISA USING HUMAN PROINSULIN-ALKALINE PHOSPHATASE CONJUGATES PREPARED BY GENE FUSION

被引：27

作者：

LINDBLADH, C ^{[1
]}

PERSSON, M ^{[1
]}

BULOW, L ^{[1
]}

STAHL, S ^{[1
]}

MOSBACH, K ^{[1
]}

机构：

[1] BIOGEN SA,CH-1211 GENEVA 24,SWITZERLAND

来源：

关键词：

D O I：

10.1016/0006-291X(87)90411-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：607 / 614

页数：8

共 17 条

[1] ATLAN D, 1987, APPL MICROBIOL BIOT, V26, P318
[2] Barman T. E, 1969, ENZYME HDB, V2
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] PREPARATION OF A SOLUBLE BIFUNCTIONAL ENZYME BY GENE FUSION
BULOW, L
LJUNGCRANTZ, P
MOSBACH, K
[J]. BIO-TECHNOLOGY, 1985, 3 (09): : 821 - 823
[5] FUSIONS OF SECRETED PROTEINS TO ALKALINE-PHOSPHATASE - AN APPROACH FOR STUDYING PROTEIN SECRETION
HOFFMAN, CS
WRIGHT, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) : 5107 - 5111
[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[7] Malamy M., 1966, METHODS ENZYMOL, V9, P639, DOI DOI 10.1016/0076-6879(66)09129-8
[8] MESSING J, 1983, METHOD ENZYMOL, V101, P20
[9] DETERMINATIONS OF PROTEINS AND HORMONES IN SERUM BY AN IMMUNOASSAY USING ANTIGEN-ENZYME CONJUGATES
MIEDEMA, K
OTTEN, JW
BOELHOUWER, J
[J]. CLINICA CHIMICA ACTA, 1972, 40 (01) : 187 - +
[10] Miller J., 1972, EXPT MOL GENETICS, P1