CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDY OF THE CATHEPSIN-B COMPLEXED WITH CA074, A SELECTIVE INHIBITOR

被引：21

作者：

YAMAMOTO, A

KAJI, T

TOMOO, K

ISHIDA, T

INOUE, M

MURATA, M

KITAMURA, K

机构：

[1] OSAKA UNIV PHARMACEUT SCI,DEPT PHYS CHEM,2-10-65 KAWAI,MATSUBARA,OSAKA 580,JAPAN

[2] TAISHO PHARMACEUT CO LTD,RES CTR,OMIYA,SAITAMA 330,JAPAN

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 227卷 / 03期

关键词：

CATHEPSIN-B; CA074; COMPLEX; CRYSTALLIZATION; PROTEIN CRYSTALLOGRAPHY;

D O I：

10.1016/0022-2836(92)90234-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cathepsin B from bovine spleen has been purified and crystallized as a complex with a specific inhibitor CA074 [N-(l-3-trans-propylcarbamoyloxirane-2-carbonyl)-l-isoleucyl-l-proline], using the hanging-drop method. The complex crystals obtained from 50 mm-citrate buffer (pH 3·5) belong to the tetragonal space group P41 (or P43) with a = 73·06 A ̊ and c = 141·59 A ̊, and diffract beyond 2·2 Å resolution. There are two complex molecules per asymmetric unit giving a packing density of 3·37 Å3/Da and indicating a high solvent content of 63·5%. © 1992.

引用

页码：942 / 944

页数：3

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[2]

DALAISSE JM, 1984, BIOCHEM BIOPH RES CO, V125, P441

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