THE PROJECTION STRUCTURE OF MICROSOMAL GLUTATHIONE TRANSFERASE

被引：32

作者：

HEBERT, H ^{[1
]}

SCHMIDTKREY, I ^{[1
]}

MORGENSTERN, R ^{[1
]}

机构：

[1] KAROLINSKA INST,INST ENVIRONM MED,DIV TOXICOL,S-17177 STOCKHOLM,SWEDEN

来源：

EMBO JOURNAL | 1995年 / 14卷 / 16期

关键词：

ELECTRON CRYSTALLOGRAPHY; MEMBRANE PROTEIN; MICROSOMAL GLUTATHIONE TRANSFERASE; 2-DIMENSIONAL CRYSTALLIZATION;

D O I：

10.1002/j.1460-2075.1995.tb00058.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Through the use of electron crystallography, it has been possible to obtain high resolution structural information regarding a mammalian protein that spans the lipid bilayer. Two-dimensional crystal of the detoxification enzyme microsomal glutathione transferase were induced by slow detergent removal from a mixture containing low amounts of phospholipid. Images of specimens stabilized in tannin were collected using electron cryomicroscopy. The projection structure at 4 Angstrom shows tightly packed trimers of the protein, Each of them contains an inner core of six parallel alpha-helices delineating a central low density region. The helical bundle is partly surrrounded by elongated domains.

引用

页码：3864 / 3869

页数：6

共 27 条

[1] FUNCTIONAL AND STRUCTURAL MEMBRANE TOPOLOGY OF RAT-LIVER MICROSOMAL GLUTATHIONE TRANSFERASE [J].

ANDERSSON, C ;

WEINANDER, R ;

LUNDQVIST, G ;

DEPIERRE, JW ;

MORGENSTERN, R .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1994, 1204 (02) :298-304

[2] CHEMICAL MODIFICATION OF RAT-LIVER MICROSOMAL GLUTATHIONE TRANSFERASE DEFINES RESIDUES OF IMPORTANCE FOR CATALYTIC FUNCTION [J].

ANDERSSON, C ;

MORGENSTERN, R .

BIOCHEMICAL JOURNAL, 1990, 272 (02) :479-484

[3]

ANDERSSON C, 1993, STRUCTURE FUNCTION G, P109

[4] GLUTATHIONE S-TRANSFERASES - REACTION-MECHANISM, STRUCTURE, AND FUNCTION [J].

ARMSTRONG, RN .

CHEMICAL RESEARCH IN TOXICOLOGY, 1991, 4 (02) :131-140

[5] IMAGES OF PURPLE MEMBRANE AT 2.8 A RESOLUTION OBTAINED BY CRYO-ELECTRON MICROSCOPY [J].

BALDWIN, JM ;

HENDERSON, R ;

BECKMAN, E ;

ZEMLIN, F .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 202 (03) :585-591

[6]

BOYER TD, 1986, J BIOL CHEM, V261, P16693

[7] FOLDING PATTERN DIVERSITY OF INTEGRAL MEMBRANE-PROTEINS [J].

COWAN, SW ;

ROSENBUSCH, JP .

SCIENCE, 1994, 264 (5161) :914-916

[8] CRYSTAL-STRUCTURES EXPLAIN FUNCTIONAL-PROPERTIES OF 2 ESCHERICHIA-COLI PORINS [J].

COWAN, SW ;

SCHIRMER, T ;

RUMMEL, G ;

STEIERT, M ;

GHOSH, R ;

PAUPTIT, RA ;

JANSONIUS, JN ;

ROSENBUSCH, JP .

NATURE, 1992, 358 (6389) :727-733

[9] STEREOCHEMISTRY OF THE MICROSOMAL GLUTATHIONE-S-TRANSFERASE CATALYZED ADDITION OF GLUTATHIONE TO CHLOROTRIFLUOROETHENE [J].

HARGUS, SJ ;

FITZSIMMONS, ME ;

ANIYA, Y ;

ANDERS, MW .

BIOCHEMISTRY, 1991, 30 (03) :717-721

[10] MODEL FOR THE STRUCTURE OF BACTERIORHODOPSIN BASED ON HIGH-RESOLUTION ELECTRON CRYOMICROSCOPY [J].

HENDERSON, R ;

BALDWIN, JM ;

CESKA, TA ;

ZEMLIN, F ;

BECKMANN, E ;

DOWNING, KH .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) :899-929

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