PRODUCTION OF A MONOCLONAL-ANTIBODY TO HUMAN-LIVER ALKALINE-PHOSPHATASE

被引：30

作者：

MEYER, LJ ^{[1
]}

LAFFERTY, MA ^{[1
]}

RADUCHA, MG ^{[1
]}

FOSTER, CJ ^{[1
]}

GOGOLIN, KJ ^{[1
]}

HARRIS, H ^{[1
]}

机构：

[1] UNIV PENN, SCH MED, DEPT HUMAN GENET, MED LABS, PHILADELPHIA, PA 19104 USA

来源：

CLINICA CHIMICA ACTA | 1982年 / 126卷 / 02期

关键词：

D O I：

10.1016/0009-8981(82)90026-2

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A monoclonal antibody to human liver alkaline phosphatase (ALP) was produced by the mouse-hybridoma method using a partially purified enzyme preparation as antigen. The particular hybridoma secreting the antibody was detected by a screening procedure based on the retention of enzyme activity by the enzyme/antibody complex. The antibody cross-reacts strongly with human kidney and bone ALP but not with human placental or intestinal ALP. It also cross-reacts with liver and kidney ALPs from gorilla, chimpanzee and orangutan. It shows no significant reaction, under the conditions used, with liver or kidney ALPs from several lower primates. An antibody affinity column was prepared and shown to be effective for the final stages of liver ALP purification.

引用

页码：109 / 117

页数：9

共 15 条

[1] PERSPECTIVES ON ALKALINE-PHOSPHATASE ISOENZYMES
FISHMAN, WH
[J]. AMERICAN JOURNAL OF MEDICINE, 1974, 56 (05) : 617 - 650
[2] ELECTROPHORESIS OF ENZYME-MONOCLONAL ANTIBODY COMPLEXES - STUDIES OF HUMAN PLACENTAL ALKALINE-PHOSPHATASE POLYMORPHISM
GOGOLIN, KJ
SLAUGHTER, CA
HARRIS, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (08): : 5061 - 5065
[3] EVOLUTION OF ALKALINE-PHOSPHATASES IN PRIMATES
GOLDSTEIN, DJ
ROGERS, C
HARRIS, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (03): : 879 - 883
[4] Harris H., 1976, Handbook of enzyme electrophoresis in human genetics
[5] LOWRY OH, 1951, J BIOL CHEM, V193, P265
[6] MCKEARN TJ, 1980, MONOCLONAL ANTIBODIE, P403
[7] COMPARISON OF HUMAN ALKALINE-PHOSPHATASE ISOENZYMES - STRUCTURAL EVIDENCE FOR 3 PROTEIN CLASSES
MCKENNA, MJ
HAMILTON, TA
SUSSMAN, HH
[J]. BIOCHEMICAL JOURNAL, 1979, 181 (01) : 67 - 73
[8] ALKALINE-PHOSPHATASE ISOENZYMES - TECHNICAL AND CLINICAL ASPECTS
MOSS, DW
[J]. ENZYME, 1975, 20 (01) : 20 - 34
[9] DEVELOPMENTAL-CHANGE IN HUMAN INTESTINAL ALKALINE-PHOSPHATASE
MULIVOR, RA
HANNIG, VL
HARRIS, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) : 3909 - 3912
[10] DIFFERENTIAL INHIBITION OF PRODUCTS OF HUMAN ALKALINE-PHOSPHATASE LOCI
MULIVOR, RA
PLOTKIN, LI
HARRIS, H
[J]. ANNALS OF HUMAN GENETICS, 1978, 42 (JUL) : 1 - 13

← 1 2 →