ANTIBODY TO BETA-AMYLOID PRECURSOR PROTEIN RECOGNIZES AN INTERMEDIATE FILAMENT-ASSOCIATED PROTEIN IN ALZHEIMERS AND CONTROL FIBROBLASTS

被引：15

作者：

DOOLEY, NP

GAUTHIER, S

DURHAM, HD

机构：

[1] MONTREAL NEUROL HOSP & INST,RM 648,3801 UNIV ST,MONTREAL H3A 2B4,QUEBEC,CANADA

[2] MCGILL UNIV,DEPT NEUROL & NEUROSURG,MONTREAL H3A 2T5,QUEBEC,CANADA

[3] MCGILL UNIV,MCGILL CTR STUDIES AGING,MONTREAL H3A 2T5,QUEBEC,CANADA

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1992年 / 33卷 / 01期

关键词：

ALZHEIMERS DISEASE; CYTOSKELETON; INTERMEDIATE FILAMENT NETWORK;

D O I：

10.1002/jnr.490330108

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The expression of beta-amyloid precursor protein (BAPP) and its mRNAs was studied in fibroblasts obtained from patients afflicted with Alzheimer's disease (AD) and age-matched controls. Using reverse transcriptase-polymerase chain reaction (RT-PCR), transcripts corresponding to 770, 751, 714, and 695 amino acids were detected in both AD and control fibroblasts. Antibody 22C11 against BAPP (Boehringer Mannheim) labeled an intracellular protein, specifically localized to the intermediate filament network. In addition to bands of the predicted molecular weights for BAPP (120-135 kDa), Western blotting revealed a 57 kDa band which was not evident in samples of human brain. As cytoskeletal elements are vital in maintaining cellular architecture and various cell interactions, localization of BAPP or a related molecule to the cytoskeleton suggests a possible structural role for this protein within the cell.

引用

收藏

页码：60 / 67

页数：8

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