CONTEXT DEPENDENCE OF HYDROGEN-BOND FREE-ENERGY REVEALED BY SUBSTITUTIONS IN AN RNA HAIRPIN

被引：204

作者：

SANTALUCIA, J

KIERZEK, R

TURNER, DH

机构：

[1] UNIV ROCHESTER,DEPT CHEM,ROCHESTER,NY 14627

[2] POLISH ACAD SCI,INST BIOORGAN CHEM,PL-60704 POZNAN,POLAND

来源：

SCIENCE | 1992年 / 256卷 / 5054期

关键词：

D O I：

10.1126/science.1373521

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Prediction and modeling of RNA structure requires knowledge of the free energy contributions of various interactions. Many unusual hydrogen bonds were recently proposed in the structure of a GCAA hairpin determined from nuclear magnetic resonance. The contributions of these hydrogen bonds to the folding stability of the hairpin formed by rGGCGCAAGCC have now been investigated through the use of functional group substitutions. These and previous results suggest a strong context dependence for the free energy of hydrogen bond formation. The results also suggest that the phylogenetic preference for GNRA (where N = A, C, G, or U and R = A or G) tetraloops may have afunctional rather than thermodynamic basis.

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页码：217 / 219

页数：3

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