INVITRO MICROPROPAGATION OF DELPHINIUM-MALABARICUM (HUTH) MUNZ - A RARE SPECIES

A micropropagation technique was developed for Delphinium malabaricum using nodes from inflorescence stalks Maximum shoot proliferation was obtained on Murashige and Skoog's medium supplemented with 2-1P (10 mg l-1) and inositol (100 mg l-1) From the sixth passage onwards, shoots could be multiplied by omission of inositol and reduction of 2-1P (0.5 mg l-1) concentration Best rooting response was obtained with a 24-h pulse treatment of shoots with 0.5 mg I-1 IBA in the dark, transfer of treated shoots to hormone-free half-strength MS medium and incubation under 24-h light. Regenerated plants were established successfully in the field Cytological examination of root tips of in vitro and control plants showed identical chromosome number (2n = 16) © 1991 Annals of Botany Company.