EXPRESSION OF FUNCTIONAL GROWTH-HORMONE RECEPTORS IN HUMAN GRANULOSA-CELLS

被引：59

作者：

CARLSSON, B ^{[1
]}

BERGH, C ^{[1
]}

BENTHAM, J ^{[1
]}

OLSSON, JH ^{[1
]}

NORMAN, MR ^{[1
]}

BILLIG, H ^{[1
]}

ROOS, P ^{[1
]}

HILLENSJO, T ^{[1
]}

机构：

[1] GOTHENBURG UNIV,DEPT OBSTET & GYNAECOL,S-41124 GOTHENBURG,SWEDEN

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 09期

关键词：

GRANULOSA CELLS; GROWTH HORMONE; GROWTH HORMONE RECEPTOR; HUMAN; STEROIDOGENESIS;

D O I：

10.1093/oxfordjournals.humrep.a137827

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Both clinical and experimental evidence suggest that growth hormone may be of importance for ovarian function. The present study investigated whether growth hormone receptors are expressed in human granulosa cells. Granulosa cells were isolated either from natural cycles or from stimulated cycles in the course of in-vitro fertilization. Total RNA hybridized with a P-32-labelled rat growth hormone receptor cRNA probe revealed one major transcript with an estimated size of 4.5 kb and one minor transcript with an estimated size of 1.3 kb. Biotinylated growth hormone was used to analyse growth hormone binding. Competitive growth hormone binding was detected in freshly isolated granulosa cells, as well as in cultured cells. Growth hormone augmented basal and/or follicle stimulating hormone-stimulated steroidogenesis in granulosa cells obtained from patients with natural cycles, but the response to growth hormone stimulation showed considerable variation. We conclude that functional growth hormone receptors are present in human granulosa cells and that growth hormone, therefore, may have an important role in ovarian function.

引用

页码：1205 / 1209

页数：5

共 32 条

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