EXPRESSION OF FUNCTIONAL GROWTH-HORMONE RECEPTORS IN HUMAN GRANULOSA-CELLS

被引：59

作者：

CARLSSON, B ^{[1
]}

BERGH, C ^{[1
]}

BENTHAM, J ^{[1
]}

OLSSON, JH ^{[1
]}

NORMAN, MR ^{[1
]}

BILLIG, H ^{[1
]}

ROOS, P ^{[1
]}

HILLENSJO, T ^{[1
]}

机构：

[1] GOTHENBURG UNIV,DEPT OBSTET & GYNAECOL,S-41124 GOTHENBURG,SWEDEN

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 09期

关键词：

GRANULOSA CELLS; GROWTH HORMONE; GROWTH HORMONE RECEPTOR; HUMAN; STEROIDOGENESIS;

D O I：

10.1093/oxfordjournals.humrep.a137827

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Both clinical and experimental evidence suggest that growth hormone may be of importance for ovarian function. The present study investigated whether growth hormone receptors are expressed in human granulosa cells. Granulosa cells were isolated either from natural cycles or from stimulated cycles in the course of in-vitro fertilization. Total RNA hybridized with a P-32-labelled rat growth hormone receptor cRNA probe revealed one major transcript with an estimated size of 4.5 kb and one minor transcript with an estimated size of 1.3 kb. Biotinylated growth hormone was used to analyse growth hormone binding. Competitive growth hormone binding was detected in freshly isolated granulosa cells, as well as in cultured cells. Growth hormone augmented basal and/or follicle stimulating hormone-stimulated steroidogenesis in granulosa cells obtained from patients with natural cycles, but the response to growth hormone stimulation showed considerable variation. We conclude that functional growth hormone receptors are present in human granulosa cells and that growth hormone, therefore, may have an important role in ovarian function.

引用

页码：1205 / 1209

页数：5

共 32 条

[11]

Erickson G.F., Garzo V.G., Magoffin D.A., Insulin-like growth factor-I regulates aromatase activity in human granulosa and granulosa luteal cells, J. Clin. Endocrinol. Metab, 69, pp. 716-724, (1989)

[12]

Gates G.S., Bayer S., Seibel M., Poretsky L., Flier J.S., Moses A.C., Characterization of insulin-like growth factor binding to human granulosa cells obtained during in vitro fertilization, J. Recept. Res, 7, pp. 885-902, (1987)

[13]

Godowski P.J., Leung D.W., Meacham L.R., Galgani J.P., Hellmiss R., Keret R., Rotwein P.S., Parks J.S., Laron Z., Wood W.I., Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism, Proc. Nall. Acad. Sci. USA, 86, pp. 8083-8087, (1989)

[14]

Homburg R., Eshel A., Abdalla H.I., Jacobs H.S., Growth hormone facilitates ovulation by gonadotrophins, Clin. Endocrinol, 29, pp. 113-117, (1988)

[15]

Hsu C.J., Hammond J.M., Concomitant effects of growth hormone on secretion of insulin-like growth factor I and progesterone by cultured porcine granulosa cells, Endocrinology, 121, pp. 1343-1348, (1987)

[16]

Hutchinson L.A., Findley J.K., Herington A.C., Growth hormone and insulin-like growth factor-I accelerate PMSG-induced differentiation of granulosa cells, Mol. Cell Endocrinol, 55, pp. 61-69, (1988)

[17]

Ibrahim Z.H.Z., Mason P.L., Buck P., Lieberman B.A., The use of biosynthetic human growth hormone to augment ovulation induction with buserelin acetate/human menopausal gonadotropin in women with a poor ovarian response, Fertil. Steril, 55, pp. 202-204, (1991)

[18]

Isaksson O.G.P., Lindahl A., Isgaard J., Action of growth hormone: Current views, Acta Pediatr. Scand, 343, pp. 12-18, (1988)

[19]

Jia X.C., Kalmijn J., Hsueh A.J., Growth hormone enhances follicle-stimulating hormone-induced differentiation of cultured rat granulosa cells, Endocrinology, 118, pp. 1401-1438, (1986)

[20]

Leung D.W., Spencer S.A., Cachianes G., Hammonds G.R., Collins C., Henzel W.J., Barnard J., Waters M.J., Wood W.I., Growth hormone receptor and serum binding protein: Purification, cloning and expression, Nature, 330, pp. 537-543, (1987)

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