CLONING AND SEQUENCING OF PROTOCHLOROPHYLLIDE REDUCTASE

被引：63

作者：

DARRAH, PM ^{[1
]}

KAY, SA ^{[1
]}

TEAKLE, GR ^{[1
]}

GRIFFITHS, WT ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1990年 / 265卷 / 03期

关键词：

D O I：

10.1042/bj2650789

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Putative protochlorophyllide reductase cDNA clones (252 and 113) were isolated from an etiolated-oat (Avena sativa) cDNA library. These were used to indirectly characterize a further clone, p127, isolated from a λ-phage gt11 cDNA library. The latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a CNBr-cleavage fragment of the purified reductase. p127 codes for more than 95% of the reductase protein.

引用

页码：789 / 798

页数：10

共 39 条

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