A THIOPHOSPHATE SUBSTRATE FOR A CONTINUOUS SPECTROPHOTOMETRIC ASSAY OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C - HEXADECYLTHIOPHOSPHORYL-1-MYO-INOSITOL

被引：18

作者：

HENDRICKSON, EK ^{[1
]}

JOHNSON, JL ^{[1
]}

HENDRICKSON, HS ^{[1
]}

机构：

[1] ST OLAF COLL, DEPT CHEM, NORTHFIELD, MN 55057 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1991年 / 1卷 / 11期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0960-894X(01)81163-X

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Racemic hexadecylthiophosphoryl-1-myo-inositol was synthesized from a pentaprotected inositol-1-dimethylphosphite and hexadecylthiophthalimide in a Michaelis-Arbuzov reaction. It was shown to be a good substrate for the assay of phosphatidylinositol-specific phospholipase C.

引用

收藏

页码：615 / +

页数：1

相关论文

共 17 条

[1] SYNTHESIS OF CHOLINE AND ETHANOLAMINE PHOSPHOLIPIDS WITH THIOPHOSPHOESTER BONDS AS SUBSTRATES FOR PHOSPHOLIPASE-C [J].

COX, JW ;

SNYDER, WR ;

HORROCKS, LA .

CHEMISTRY AND PHYSICS OF LIPIDS, 1979, 25 (04) :369-380

[2] SYNTHESIS OF 1-0-(1,2-DI-0-PALMITOYL-SN-GLYCERO-3-PHOSPHO)-D-MYO-INOSITOL 4,5-BISPHOSPHATE - AN ANALOG OF NATURALLY-OCCURRING (PTD)INS(4,5)P2 [J].

DREEF, CE ;

ELIE, CJJ ;

HOOGERHOUT, P ;

VANDERMAREL, GA ;

VANBOOM, JH .

TETRAHEDRON LETTERS, 1988, 29 (49) :6513-6516

[3] SYNTHESIS OF SOME MONO-O-BENZYL-MYO-INOSITOL AND PENTA-O-METHYL-MYO-INOSITOL [J].

GAREGG, PJ ;

IVERSEN, T ;

JOHANSSON, R ;

LINDBERG, B .

CARBOHYDRATE RESEARCH, 1984, 130 (JUL) :322-326

[4]

GRIFFITH OH, 1991, METHOD ENZYMOL, V197, P493

[5]

HENDRICKSON HS, 1983, J LIPID RES, V24, P1532

[6]

HENDRICKSON HS, 1990, CHEM PHYS LIPIDS, V53, P115

[7]

HENDRICKSON HS, 1984, J BIOL CHEM, V259, P5734

[8]

Ikezawa H., 1981, METHODS ENZYMOLOGY C, V71, P731, DOI [DOI 10.1016/0076-6879(81)71086-3, 10.1016/0076-6879(81)71086-3]

[9] IMPROVED SYNTHESES OF INOSITOL PHOSPHOLIPID ANALOGS [J].

JONES, M ;

RANA, KK ;

WARD, JG ;

YOUNG, RC .

TETRAHEDRON LETTERS, 1989, 30 (39) :5353-5356

[10] PHOSPHOLIPIDS CHIRAL AT PHOSPHORUS - STEREOCHEMICAL MECHANISM OF REACTIONS CATALYZED BY PHOSPHATIDYLINOSITIDE-SPECIFIC PHOSPHOLIPASE-C FROM BACILLUS-CEREUS AND GUINEA-PIG UTERUS [J].

LIN, G ;

BENNETT, CF ;

TSAI, MD .

BIOCHEMISTRY, 1990, 29 (11) :2747-2757