STABILITY OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COLUMNS PACKED WITH C-1 AND C-8 POLYSILOXANES SORBED INTO POROUS SILICA PARTICLES

被引：13

作者：

ANAZAWA, TA ^{[1
]}

CARRARO, F ^{[1
]}

COLLINS, KE ^{[1
]}

JARDIM, ICSF ^{[1
]}

机构：

[1] UNIV ESTADUAL CAMPINAS,INST QUIM,BR-13083970 CAMPINAS,BRAZIL

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 697卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)00810-V

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Columns, packed with 10 mu m diameter porous silica particles (15 nm diameter pores) containing polydimethylsiloxane (20%, w/w) or polymethyloctylsiloxane (40%, w/w) sorbed into the pores, were tested for chromatographic stability as a function of elution volume. No significant changes in efficiency, retention factor or separation factor were observed following extended washing (up to 5000 column volumes) of either stationary phase with methanol-water mobile phases.

引用

页码：159 / 164

页数：6

共 6 条

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[2]

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[3]

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[4]

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[5]

Schmit, Modern Practice of Liquid Chromatography, (1971)

[6]

Anazawa, Jardim, J. Liq. Chromatogr., 17, (1994)

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