INOSITOL TRISPHOSPHATE AND THAPSIGARGIN DISCRIMINATE ENDOPLASMIC-RETICULUM STORES OF CALCIUM IN RAT-BRAIN

被引：93

作者：

VERMA, A

HIRSCH, DJ

HANLEY, MR

THASTRUP, O

CHRISTENSEN, SB

SNYDER, SH

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,75 N WOLFE ST,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT,BALTIMORE,MD 21205

[4] UNIV CALIF DAVIS,SCH MED,DEPT BIOL CHEM,DAVIS,CA 95616

[5] UNIV COPENHAGEN,RIKSHOSP,DEPT CLIN CHEM,DK-2100 COPENHAGEN,DENMARK

[6] ROYAL DANISH SCH PHARM,DEPT ORGAN CHEM,DK-2100 COPENHAGEN,DENMARK

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90747-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 μM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3. © 1990.

引用

页码：811 / 816

页数：6

共 16 条

[1] CALCIUM-SENSITIVITY AND INOSITOL POLYPHOSPHATE-SENSITIVITY OF THE CALCIUM-SEQUESTERING ENDOPLASMIC-RETICULUM IN THE PHOTORECEPTOR CELLS OF THE HONEYBEE DRONE [J].