DIFFERENTIAL-EFFECTS OF BCL-2 ON T-CELLS AND B-CELLS IN TRANSGENIC MICE

被引：123

作者：

KATSUMATA, M ^{[1
]}

SIEGEL, RM ^{[1
]}

LOUIE, DC ^{[1
]}

MIYASHITA, T ^{[1
]}

TSUJIMOTO, Y ^{[1
]}

NOWELL, PC ^{[1
]}

GREENE, MI ^{[1
]}

REED, JC ^{[1
]}

机构：

[1] WISTAR INST,PHILADELPHIA,PA 19104

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 23期

关键词：

GENETICS OF LYMPHOMA; APOPTOSIS; T-CELL DEVELOPMENT; T-CELL SUBSETS; ONCOGENE;

D O I：

10.1073/pnas.89.23.11376

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have produced bcl-2 transgenic mice by using a construct which mimics the t(14;18) translocation in human follicular lymphomas. Although lymphoid tissues from all transgenic mice contained high levels of human Bcl-2 protein, transgene expression was differentially regulated within the B- and T-cell compartments of lines derived from various founder mice. We have characterized the phenotypes of two lines of bcl-2 transgenic mice (line 2 and line 6) in which bcl-2 transgene expression was restricted primarily to the T- or B-cell lineages, respectively. Analysis of line 6 lymphocytes revealed a polyclonal expansion of B cells, and these B cells exhibited prolonged survival in vitro. In line 2 mice, numbers of T cells in the peripheral lymphoid tissues were more moderately elevated despite enhanced T-cell survival in vitro. Line 2 transgenic mice also showed significantly increased proportions of thymocytes with a mature phenotype. Taken together, these findings suggest different roles for bcl-2 in the in vivo regulation of B- and T-cell development and homeostasis.

引用

页码：11376 / 11380

页数：5

共 22 条

[11]

REED JC, 1992, IN PRESS LAB INVEST

[12] BCL-2 INHIBITS MULTIPLE FORMS OF APOPTOSIS BUT NOT NEGATIVE SELECTION IN THYMOCYTES [J].