APPRAISAL OF FLUORESCENCE IN-SITU HYBRIDIZATION (FISH) TECHNIQUES IN PRENATAL-DIAGNOSIS

被引：4

作者：

GRAO, P ^{[1
]}

GUITART, M ^{[1
]}

COLL, MD ^{[1
]}

EGOZCUE, J ^{[1
]}

机构：

[1] HOSP SABADELL,DEPT GENET,BARCELONA,SPAIN

来源：

EARLY HUMAN DEVELOPMENT | 1993年 / 33卷 / 02期

关键词：

IN-SITU HYBRIDIZATION; AMNIOCYTES; CHORIONIC VILLI; PROBES;

D O I：

10.1016/0378-3782(93)90205-9

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The reliability of FISH was appraised using probes for the X and Y chromosome and for chromosomes 12 and 18 in prenatal and adult interphase nuclei. Detection of a single hybridization spot proved to be quite reliable (80-92% positive nuclei). Detection of two hybridization spots was more difficult; percentages of nuclei showing two signals varied between 62-72%. The percentages of nuclei with the correct number of spots was higher in the metaphases occasionally found. Thus, FISH may complement but not replace cytogenetic analysis. For sex determination and for the detection of mosaicism, we suggest the use of two different probes in separate regions of the same preparation.

引用

页码：101 / 108

页数：8

共 20 条

[1] RAPID INTERPHASE AND METAPHASE ASSESSMENT OF SPECIFIC CHROMOSOMAL CHANGES IN NEUROECTODERMAL TUMOR-CELLS BY INSITU HYBRIDIZATION WITH CHEMICALLY MODIFIED DNA PROBES [J].