MATERIALS FOR ENHANCING CELL-ADHESION BY IMMOBILIZATION OF CELL-ADHESIVE PEPTIDE

被引：109

作者：

ITO, Y ^{[1
]}

KAJIHARA, M ^{[1
]}

IMANISHI, Y ^{[1
]}

机构：

[1] KYOTO UNIV,DEPT POLYMER CHEM,YOSHIDA HONMACHI,SAKYO KU,KYOTO 606,JAPAN

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1991年 / 25卷 / 11期

关键词：

D O I：

10.1002/jbm.820251102

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Materials to enhance cell adhesion were synthesized by surface integration of peptide, Arg-Gly-Asp-Ser(RGDS), which is an active-site sequence of cell-adhesive proteins. Polystyrene film was glow-discharged and graft-copolymerized with acrylic acid. Then the peptide was immobilized to the poly(acrylic acid) grafts by using water-soluble carbodiimide. The cell-adhesive activity of the RGDS-immobilized film increased with increasing amount of immobilized peptide, and approached the activity of fibronectin(FN)-immobilized film. The RGDS-immobilized film was more stable against heat treatment and pH variation than the FN-immobilized film. In addition, the RGDS-immobilized film enhanced cell growth more strongly than the FN-immobilized film.

引用

页码：1325 / 1337

页数：13

共 37 条

[1]

AKIYAMA SK, 1987, ADV ENZYMOL, V57, P1

[2] ORTHO-PHTHALALDEHYDE - FLUOROGENIC DETECTION OF PRIMARY AMINES IN PICOMOLE RANGE - COMPARISON WITH FLUORESCAMINE AND NINHYDRIN [J].

BENSON, JR ;

HARE, PE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (02) :619-622

[3] COVALENT ATTACHMENT OF AN ARG-GLY-ASP SEQUENCE PEPTIDE TO DERIVATIZABLE POLYACRYLAMIDE SURFACES - SUPPORT OF FIBROBLAST ADHESION AND LONG-TERM GROWTH [J].

BRANDLEY, BK ;

SCHNAAR, RL .

ANALYTICAL BIOCHEMISTRY, 1988, 172 (01) :270-278

[4] PREVENTION OF ERYTHROCYTE ADHESION ONTO POROUS SURFACES BY FLUID PERFUSION [J].

CLARKE, DR ;

PARK, JB .

BIOMATERIALS, 1981, 2 (01) :9-13

[5]

CURTIS ASG, 1984, J CELLS CI, V86, P9

[6] (ARG-GLY-ASP)N-ALBUMIN CONJUGATES AS A MODEL SUBSTRATUM FOR INTEGRIN-MEDIATED CELL-ADHESION [J].

DANILOV, YN ;

JULIANO, RL .

EXPERIMENTAL CELL RESEARCH, 1989, 182 (01) :186-196

[7] ADSORPTION CHARACTERISTICS OF PLASMA FIBRONECTIN IN RELATIONSHIP TO BIOLOGICAL-ACTIVITY [J].

GRINNELL, F ;

FELD, MK .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1981, 15 (03) :363-381

[8] MORPHOLOGY OF BLOCK POLYMERS NEAR A FREE-SURFACE [J].

HASEGAWA, H ;

HASHIMOTO, T .

MACROMOLECULES, 1985, 18 (03) :589-590

[9] SIMS AND XPS STUDIES OF POLYURETHANE SURFACES .1. PRELIMINARY STUDIES [J].

HEARN, MJ ;

RATNER, BD ;

BRIGGS, D .

MACROMOLECULES, 1988, 21 (10) :2950-2959

[10] CELL-ADHESION TO A SERIES OF HYDROPHILIC-HYDROPHOBIC COPOLYMERS STUDIED WITH A SPINNING DISK APPARATUS [J].

HORBETT, TA ;

WALDBURGER, JJ ;

RATNER, BD ;

HOFFMAN, AS .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1988, 22 (05) :383-404

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