MOLECULAR-CLONING OF A NEW CLASS OF CARTILAGE-SPECIFIC MATRIX, CHONDROMODULIN-I, WHICH STIMULATES GROWTH OF CULTURED CHONDROCYTES

被引：111

作者：

HIRAKI, Y ^{[1
]}

TANAKA, H ^{[1
]}

INOUE, H ^{[1
]}

KONDO, J ^{[1
]}

KAMIZONO, A ^{[1
]}

SUZUKI, F ^{[1
]}

机构：

[1] MITSUBISHI KASEI CORP,RES CTR,MIDORI KU,YOKOHAMA 227,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91660-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report the structure and bioactivity of 25 kDa glycoprotein (chondromodulin-I) as a tissue-specific functional matrix component identified and cloned for the first time. Chondromodulin-I purified from fetal bovine cartilage markedly stimulated DNA synthesis of cultured growth-plate chondrocytes in the presence of basic fibroblast growth factor (FGF). Bovine chondromodulin-I cDNA revealed that the mature protein consists of 121 amino acids with three possible glycosylation sites and is coded as the C-terminal part of a larger precursor. On northern blot analysis, expression of chondromodulin-I mRNA was observed only in cartilage. © 1991.

引用

页码：971 / 977

页数：7

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