MONOCLONAL-ANTIBODIES AGAINST THE PLANT CYTOKININ, CIS-ZEATIN RIBOSIDE

被引：4

作者：

BANOWETZ, GM

机构：

[1] USDA ARS, Corvallis, OR 97331

来源：

HYBRIDOMA | 1993年 / 12卷 / 06期

关键词：

D O I：

10.1089/hyb.1993.12.729

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three monoclonal antibodies (two IgG1 and one IgA) prepared against the plant cytokinin, cis-zeatin riboside, were characterized for use in a fluorescence competition enzyme linked immunosorbant assay (F-ELISA). Immunoassays that employed each antibody detected femtomole quantities of the homologous cytokinin and characteristic quantities of structurally-related cytokinins. One of the antibodies was used to quantify HPLC-purified cis- zeatin riboside recovered from spiked samples of wheat leaves. These are the first monoclonal antibodies against a cis- derivative of a plant cytokinin and will be useful for purification and quantification of cis-zeatin and the 9-riboside and 9-glucoside in plant samples.

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页码：729 / 736

页数：8

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