PLATELET-ACTIVATING-FACTOR ACETYLHYDROLASES IN CACO-2 CELLS AND EPITHELIUM OF NORMAL AND ULCERATIVE-COLITIS PATIENTS

被引:9
作者
RIEHL, T [1 ]
STENSON, WF [1 ]
机构
[1] WASHINGTON UNIV,SCH MED,DEPT MED,ST LOUIS,MO 63110
关键词
D O I
10.1016/0016-5085(95)90749-1
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background & Aims: Platelet-activating factor (PAF) is a potent inflammatory mediator implicated in the pathogenesis of inflammatory bowel disease and necrotizing enterocolitis, Metabolism by platelet-activating factor acetylhydrolase (PAF-AH) is the major pathway for platelet-activating factor degradation. The aim of this study was to investigate the possible role of intestinal epithelium as a source of PAF-AH, Methods: Intracellular and secreted PAF-AHs were characterized in human colonic epithelial cells isolated from histologically normal mucosa and inflamed mucosa from patients with ulcerative colitis and in the human intestinal epithelial cell line Caco-2 by measuring the metabolism of [H-3]PAF to [H-3]lysoPAF. Results: Human colonic epithelial cells and Caco-2 cells synthesize and secrete PAF-AH as shown by in vitro hydrolysis of [H-3]PAF to [H-3]lysoPAF in cell lysates and conditioned media, Both intracellular and secreted PAF-AHs are calcium-independent and substrate-specific for phospholipids similar to PAF, Epithelial cells from involved areas of resections for ulcerative colitis had increased levels of secreted PAF-AH and decreased levels of intracellular PAF-AH compared with epithelial cells from histologically normal areas. Conclusions: Human colonic epithelial cells and Caco-2 cells produce intracellular and secreted PAF-AHs, which are distinct proteins. This is the first demonstration of PAF-AH production by epithelial cells.
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页码:1826 / 1834
页数:9
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