CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF THIOESTERASE-II FROM RAT MAMMARY-GLAND

被引：6

作者：

BUCHBINDER, JL

WITKOWSKI, A

SMITH, S

FLETTERICK, RJ

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

[2] CHILDRENS HOSP,OAKLAND RES INST,OAKLAND,CA 94609

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1995年 / 22卷 / 01期

关键词：

THIOESTERASE; CRYSTALLIZATION; X-RAY DIFFRACTION;

D O I：

10.1002/prot.340220111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thioesterase II from rat mammary gland has been crystallized in the presence of decanoic acid by the vapor diffusion method. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), and have cell dimensions, a = 52.7 Angstrom, b = 78.0 Angstrom, and c = 133.6 Angstrom. The asymmetric unit likely consists of two protein monomers based on predictions from its calculated Matthews coefficient. Crystals typically diffract to at least 2.5 Angstrom resolution and are suitable for X-ray crystallographic analysis. (C) 1995 Wiley-Liss, Inc.

引用

页码：73 / 75

页数：3

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