PRODUCTION OF HUMAN INTERLEUKIN-3 USING INDUSTRIAL MICROORGANISMS

被引：38

作者：

VANLEEN, RW

BAKHUIS, JG

VANBECKHOVEN, RFWC

BURGER, H

DORSSERS, LCJ

HOMMES, RWJ

LEMSON, PJ

NOORDAM, B

PERSOON, NLM

WAGEMAKER, G

机构：

[1] TNO,ITRI,EXPTL HEMATOL SECT,RIJSWIJK,NETHERLANDS

[2] DR DANIEL DENHOED CLIN,3008 EA ROTTERDAM,NETHERLANDS

[3] ERASMUS UNIV,INST RADIOBIOL,3000 DR ROTTERDAM,NETHERLANDS

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 01期

关键词：

D O I：

10.1038/nbt0191-47

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We expressed a cDNA encoding the multi-colony stimulating factor interleukin-3 in a variety of cell types, including bacteria, yeast and mammalian cells. After evaluation of the advantages and disadvantages of each potential system, we designed a production and purification scheme using Bacillus licheniformis. The purification consists of hydrophobic interaction chromatography, two steps of ion exchange chromatography and gel filtration. The purified and formulated product entered clinical trials in November 1989.

引用

页码：47 / 52

页数：6

共 28 条

[1]

BOYD A, 1989, MICROBIAL PRODUCTS N, P271

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