OPTIMIZED METHOD FOR THE DETERMINATION OF 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE AND 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE MOLECULAR-SPECIES BY ENZYMATIC-HYDROLYSIS AND GAS-CHROMATOGRAPHY

被引：13

作者：

OLSSON, NU

KAUFMANN, P

机构：

[1] Karlshamns LipidTeknik AB, S-104 65 Stockholm

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 600卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85556-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method was developed for the analysis of molecular species of 1,2-diacyl-sn-3-glycerophosphocholine and 1,2-diacyl-sn-3-glycerol-phosphoethanolamine from natural sources. The method involves enzymatic hydrolysis of the sn-3 position of the glycerophospho-lipids, acetylation of the newly liberated hydroxyl group and high-temperature gas chromatography of the 1,2-diacyl-3-acetyl-sn-glycerols. Both the enzymatic hydrolysis and the gas chromatographic separation were optimized with chemometric methods.

引用

页码：257 / 266

页数：10

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