INCREASED SENSITIVITY OF WESTERN BLOTS USING VARIOUS SECONDARY ANTIBODY DETECTION METHODS

被引：6

作者：

JEFFERSON, WN ^{[1
]}

VOELKER, CR ^{[1
]}

NEWBOLD, RR ^{[1
]}

机构：

[1] NIEHS,DIV INTRAMURAL RES,REPRODUCT & DEV TOXICOL LAB,POB 12233,RES TRIANGLE PK,NC 27709

来源：

TOXICOLOGY METHODS | 1993年 / 3卷 / 02期

关键词：

WESTERN BLOT; IMMUNOGOLD; I-125; BIOTINYLATION; ENHANCED CHEMILUMINESCENCE;

D O I：

10.3109/15376519309047234

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The sensitivity of Western blots using four different secondary antibody detection methods is described and compared. Enhanced chemiluminescence (ECL) and biotinylation are fast and offer many advantages over the other two described methods. Both ECL and biotinylation provide a linear response to increasing known amounts of proteins measured by quantitative analysis. However, ECL was the most sensitive method tested; in addition to an excellent linear response, ECL allowed multiple exposures of the same blot. In summary, ECL is highly sensitive, safe, and fast. This detection method should prove useful for a wide array of applications.

引用

页码：100 / 108

页数：9

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