A MICROFLUOROMETRIC METHOD FOR SIMULTANEOUS MEASUREMENT OF CHANGES IN CYTOSOLIC FREE CALCIUM-CONCENTRATION AND PH IN SINGLE CARDIAC MYOCYTES

被引：16

作者：

SHIBUYA, I

MATSUYAMA, K

TANAKA, K

DOI, K

机构：

[1] Department of Physiology, Yamagata University School of Medicine

来源：

JAPANESE JOURNAL OF PHYSIOLOGY | 1991年 / 41卷 / 02期

关键词：

CYTOSOLIC FREE CALCIUM CONCENTRATION; CYTOSOLIC PH; MICROFLUOROMETRY;

D O I：

10.2170/jjphysiol.41.341

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We describe a new system to measure changes in cytosolic free Ca concentration ([Ca2+]i) and pH (pHi) simultaneously using the dyes fura-2 and BCECF, and a random access camera with computer-operated synchronously-changing excitation and emission filters. Fluorescence intensities for fura-2 and BCECF were recorded from rat cardiac myocytes and calibrated in vivo using multiple-point determinations. [Ca2+]i and pHi were 88.6 +/- 32.8 nM and 7.16 +/- 0.12, respectively.

引用

收藏

页码：341 / 350

页数：10

相关论文

共 17 条

[1] MECHANISM OF BASOLATERAL MEMBRANE H+/OH-/HCO3- TRANSPORT IN THE RAT PROXIMAL CONVOLUTED TUBULE - A SODIUM-COUPLED ELECTROGENIC PROCESS [J].

ALPERN, RJ .

JOURNAL OF GENERAL PHYSIOLOGY, 1985, 86 (05) :613-636

[2] INHIBITION OF THE ACTIVATION AND TROPONIN CALCIUM-BINDING OF DOG CARDIAC MYOFIBRILS BY ACIDIC PH [J].

BLANCHARD, EM ;

SOLARO, RJ .

CIRCULATION RESEARCH, 1984, 55 (03) :382-391

[3] INSITU CALIBRATION OF FURA-2 AND BCECF FLUORESCENCE IN ADULT-RAT VENTRICULAR MYOCYTES [J].

BORZAK, S ;

KELLY, RA ;

KRAMER, BK ;

MATOBA, Y ;

MARSH, JD ;

REERS, M .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (03) :H973-H981

[4] EFFECT OF INTRACELLULAR AND EXTRACELLULAR PH ON CONTRACTION IN ISOLATED, MAMMALIAN CARDIAC-MUSCLE [J].

BOUNTRA, C ;

VAUGHANJONES, RD .

JOURNAL OF PHYSIOLOGY-LONDON, 1989, 418 :163-187

[5] THE EFFECTS OF METABOLIC INHIBITION ON INTRACELLULAR CALCIUM AND PH IN ISOLATED RAT VENTRICULAR CELLS [J].

EISNER, DA ;

NICHOLS, CG ;

ONEILL, SC ;

SMITH, GL ;

VALDEOLMILLOS, M .

JOURNAL OF PHYSIOLOGY-LONDON, 1989, 411 :393-418

[6]

FUJIMOTO M, 1989, JPN J PHYSL, V39, P273

[7]

GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440

[8] INTRACELLULAR AND EXTRACELLULAR ACTIONS OF PROTON ON THE CALCIUM CURRENT OF ISOLATED GUINEA-PIG VENTRICULAR CELLS [J].

IRISAWA, H ;

SATO, R .

CIRCULATION RESEARCH, 1986, 59 (03) :348-355

[9]

LI QA, 1987, BIOCHEM BIOPH RES CO, V147, P120

[10] DUAL LOADING OF THE FLUORESCENT INDICATOR FURA-2 AND 2,7-BISCARBOXYETHYL-5(6)-CARBOXYFLUORESCEIN (BCECF) IN ISOLATED MYOCYTES [J].

MIYATA, H ;

HAYASHI, H ;

SUZUKI, S ;

NODA, N ;

KOBAYASHI, A ;

FUJIWAKE, H ;

HIRANO, M ;

YAMAZAKI, N .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 163 (01) :500-505