THE SPINACH CHLOROPLAST CHROMOSOME IS BOUND TO THE THYLAKOID MEMBRANE IN THE REGION OF THE INVERTED REPEAT

被引：27

作者：

LIU, JW

ROSE, RJ

机构：

[1] Department of Biological Sciences, The University of Newcastle

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90689-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using a novel fractionation procedure we show that the chloroplast chromosome is specifically bound to the spinach thylakoid in the region of the inverted repeat, near the 16S and 23S rRNA genes. Central to the method is the use of restriction endonucleases, followed by ethylenediaminetetraacetic acid (EDTA) treatment to loosen or uncoil the nucleoid by the removal of specific proteins. The data we present provide a basis for understanding the molecular mechanism of membrane-bound cpDNA function. © 1992.

引用

页码：993 / 1000

页数：8

共 18 条

[1] ISOLATION OF A PROTEIN SCAFFOLD FROM MITOTIC HELA-CELL CHROMOSOMES [J].