PURIFICATION PROPERTIES OF 2 CHITINASES FROM STREPTOMYCES SP J-13-3

被引：23

作者：

OKAZAKI, K ^{[1
]}

KATO, F ^{[1
]}

WATANABE, N ^{[1
]}

YASUDA, S ^{[1
]}

MASUI, Y ^{[1
]}

HAYAKAWA, S ^{[1
]}

机构：

[1] OTSUKA PHARMACEUT CO LTD, INST CELLULAR TECHNOL, TOKUSHIMA 77101, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 08期

关键词：

D O I：

10.1271/bbb.59.1586

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two chitinases (Chi-A and Chi-B) purified from Streptomyces sp. J-13-3 had the same molecular weights (31,000) and enzymatic properties (optimum pH and temperature of pH 6.0 and 45 degrees C) hut bad significantly different isoelectric points (3.9 for Chi-A, 3.5 for Chi-B). Chi-A and -B had identical N-terminal amino acid sequences (ADXAAAWNASSVYTGGGSASYNGHN), similar amino acid compositions, and immunological cross-reactivities. A concomitant decrease of Chi-A and increase of Chi-B was observed in their productions during cultivation.

引用

页码：1586 / 1587

页数：2

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