THE ABILITY OF MEMBRANE-POTENTIAL DYES AND CALCAFLUOR WHITE TO DISTINGUISH BETWEEN VIABLE AND NONVIABLE BACTERIA

被引：96

作者：

MASON, DJ ^{[1
]}

LOPEZAMOROS, R ^{[1
]}

ALLMAN, R ^{[1
]}

STARK, JM ^{[1
]}

LLOYD, D ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF, SCH PURE & APPL BIOL, CARDIFF CF1 3TL, S GLAM, WALES

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 78卷 / 03期

关键词：

D O I：

10.1111/j.1365-2672.1995.tb05031.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.

引用

页码：309 / 315

页数：7

共 27 条

[1] A RAPID ANALYTICAL TECHNIQUE FOR FLOW CYTOMETRIC ANALYSIS OF CELL VIABILITY USING CALCOFLUOR WHITE M2R
BERGLUND, DL
TAFFS, RE
ROBERTSON, NP
[J]. CYTOMETRY, 1987, 8 (04): : 421 - 426
[2] COMPARATIVE MEASUREMENTS OF MEMBRANE-POTENTIALS WITH MICROELECTRODES AND VOLTAGE-SENSITIVE DYES
BRAUNER, T
HULSER, DF
STRASSER, RJ
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 771 (02) : 208 - 216
[3] Carter Elizabeth A., 1993, P111
[4] ABSORPTION AND TRANSPORT OF FLUORESCENT BRIGHTENERS BY MICROORGANISMS
DARKEN, MA
[J]. APPLIED MICROBIOLOGY, 1962, 10 (05) : 387 - +
[5] Davey Hazel M., 1993, P83
[6] DIAPER JP, 1992, APPL MICROBIOL BIOT, V22, P1
[7] EDDY AA, 1989, METHOD ENZYMOL, V172, P95
[8] SIMULTANEOUS IMAGING OF CELL AND MITOCHONDRIAL-MEMBRANE POTENTIALS
FARKAS, DL
WEI, MD
FEBBRORIELLO, P
CARSON, JH
LOEW, LM
[J]. BIOPHYSICAL JOURNAL, 1989, 56 (06) : 1053 - 1069
[9] DETERMINATION OF THE MEMBRANE-POTENTIAL OF CULTURED MAMMALIAN SCHWANN-CELLS AND ITS SENSITIVITY TO POTASSIUM USING A THIOCARBOCYANINE FLUORESCENT DYE
HARGITTAI, PT
YOUMANS, SJ
LIEBERMAN, EM
[J]. GLIA, 1991, 4 (06) : 611 - 616
[10] RAPID ASSESSMENT OF BACTERIAL VIABILITY AND VITALITY BY RHODAMINE 123 AND FLOW-CYTOMETRY
KAPRELYANTS, AS
KELL, DB
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (05): : 410 - 422

← 1 2 3 →