CAPILLARY ELECTROPHORETIC DETERMINATION OF S-CARBOXYMETHYL-L-CYSTEINE AND ITS MAJOR METABOLITES IN HUMAN URINE - FEASIBILITY INVESTIGATION USING ON-COLUMN DETECTION OF NON-DERIVATIZED SOLUTES IN CAPILLARIES WITH MINIMAL ELECTROOSMOSIS

被引：26

作者：

TANAKA, Y ^{[1
]}

THORMANN, W ^{[1
]}

机构：

[1] UNIV BERN,DEPT CLIN PHARMACOL,MURTENSTR 35,CH-3010 BERN,SWITZERLAND

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 09期

关键词：

D O I：

10.1002/elps.1150110914

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The capillary electrophoretic analysis of S‐carboxymethyl‐L‐cysteine and some of its metabolites in human urine is reported using (i) on‐column detection of underivatized solutes, (ii) minimal sample pretreatment and (iii) capillary columns with minimized electroosmosis. Experimental results obtained with two apparatus, the HPE‐100 (Bio‐Rad), featuring coated fused silica capillaries of 25 μm i.d., and with the Tachophor 2127 (LKB), having Teflon capillaries of 500 μm i.d. are discussed. Drug concentrations down to 0.2 mg/mL on capillary isotachophoresis and 0.03 mg/mL on capillary zone electrophoresis can be monitored with these instruments. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：760 / 764

页数：5

共 12 条

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