ELECTROSPRAY MASS-SPECTROMETRIC CHARACTERIZATION OF FLUOROQUINOLONE ANTIBIOTICS - NORFLOXACIN, ENOXACIN, CIPROFLOXACIN AND OFLOXACIN

被引:19
作者
DAGOSTINO, PA
HANCOCK, JR
PROVOST, LR
机构
[1] Defence Research Establishment Suffield, Medicine Hat, Alberta, T1A 8K6
关键词
D O I
10.1002/rcm.1290091114
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report the electrospray (ESI) mass spectra of four fluoroquinolones, enoxacin, norfloxacin, ofloxacin and ciprofloxacin, a group of highly potent antibiotics that have not been previously characterized by this ionization technique. At low sampling cone voltage settings, 50 and 100 V, the electrospray mass spectra contained primarily molecular adduct ions. At a sampling cone voltage setting of 150 V, collisionally activated dissociation mass spectra containing structurally significant product ions were produced and accurately mass measured at a magnetic sector resolution of 3000. Gradient separation and characterization of these compounds was demonstrated by analysis using packed capillary column liquid chromatography with UV and ESI-MS detection. UV detection limits in the 1 to 2 ng range ((signal-to-noise ratio S/N) = 10:1) were estimated and interpretable mass spectra with S/N ratios in excess of 100:1 were observed for 1.5 ng of analyte during electrospray mass spectrometric analysis.
引用
收藏
页码:1038 / 1043
页数:6
相关论文
共 17 条
[1]   MASS-SPECTROMETRY OF PEPTIDES AND PROTEINS [J].
BIEMANN, K .
ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :977-1010
[2]  
BURLINGAME AL, 1994, ANAL CHEM, V66, pR634
[3]   UNEXPECTED BEHAVIOR OF QUINOLONE SULFAMATES BY VARIOUS MASS-SPECTROMETRIC IONIZATION TECHNIQUES [J].
CEPA, SP ;
HILL, P ;
BUKO, AM .
BIOLOGICAL MASS SPECTROMETRY, 1992, 21 (03) :176-178
[4]   CLASS-SELECTIVE COLLISIONALLY ACTIVATED DISSOCIATION ION-MOLECULE REACTIONS OF 4-QUINOLONE ANTIBIOTICS [J].
COLORADO, A ;
BRODBELT, J .
ANALYTICAL CHEMISTRY, 1994, 66 (14) :2330-2335
[5]  
DAGOSTINO PA, 1995, 43RD ANN C MASS SPEC
[6]   ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
SCIENCE, 1989, 246 (4926) :64-71
[7]  
GAU W, 1986, ARZNEIMITTEL-FORSCH, V36-2, P1545
[8]  
HIDEKI H, 1993, HOCHUDOKU, V11, P166
[9]  
HOOPER DC, 1993, QUINOLONE ANTIMICROB
[10]   HIGH-FLOW ION SPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY [J].
HOPFGARTNER, G ;
WACHS, T ;
BEAN, K ;
HENION, J .
ANALYTICAL CHEMISTRY, 1993, 65 (04) :439-446