SIMPLIFIED COPURIFICATION OF VASCULAR SMOOTH-MUSCLE CALPONIN AND CALDESMON

被引：27

作者：

ABE, M

TAKAHASHI, K

HIWADA, K

机构：

[1] Second Department of Internal Medicine, Ehime University School of Medicine, Onsen-gun

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 107卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the co-purification of calponin and caldesmon from bovine aorta smooth muscle was established involving heat treatment, ammonium sulfate fractionation (0-30 and 30-50%), CM52 column chromatography, and Ultrogel AcA44 gel filtration. The yields of calponin and caldesmon from 200 g of smooth muscle were 17 and 32 mg, respectively. This simplified, fast, and efficient method for the purification of calponin and caldesmon constitutes a useful tool for studying thin filament-linked regulation of smooth muscle contraction. © 1990 COPYRIGHT, 1990 BY The Journal of Biochemistry.

引用

页码：507 / 509

页数：3

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