INHIBITION OF PCR-BASED ASSAY FOR BORDETELLA-PERTUSSIS BY USING CALCIUM ALGINATE FIBER AND ALUMINUM SHAFT COMPONENTS OF A NASOPHARYNGEAL SWAB

被引：67

作者：

WADOWSKY, RM

LAUS, S

LIBERT, T

STATES, SJ

EHRLICH, GD

机构：

[1] CITY PITTSBURGH WATER DEPT,PITTSBURGH,PA 15215

[2] CHILDRENS HOSP PITTSBURGH,DEPT PEDIAT OTOLARYNGOL,PITTSBURGH,PA 15213

[3] CHILDRENS HOSP PITTSBURGH,CLIN MICROBIOL LAB,PITTSBURGH,PA 15213

[4] UNIV PITTSBURGH,SCH MED,DEPT PATHOL,PITTSBURGH,PA 15261

[5] UNIV PITTSBURGH,SCH MED,DEPT OTOLARYNGOL,PITTSBURGH,PA 15261

[6] UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT INFECT DIS & MICROBIOL,PITTSBURGH,PA 15261

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 04期

关键词：

D O I：

10.1128/JCM.32.4.1054-1057.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.

引用

页码：1054 / 1057

页数：4

共 14 条

[1]

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