CHARACTERIZATION OF DISTINCT ALPHA-TYPE AND GAMMA-TYPE GLIADINS AND LOW-MOLECULAR-WEIGHT COMPONENTS FROM WHEAT ENDOSPERM AS CELIAC IMMUNOREACTIVE PROTEINS

被引：23

作者：

ROCHER, A ^{[1
]}

SORIANO, F ^{[1
]}

MOLINA, E ^{[1
]}

GONZALEZLIMAS, G ^{[1
]}

MENDEZ, E ^{[1
]}

机构：

[1] HOSP RAMON Y CAJAL, SERV ENDOCRINOL, E-28034 MADRID, SPAIN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1995年 / 1247卷 / 01期

关键词：

GLIADIN; CELIAC DISEASE; HPLC; IMMUNOBLOTTING;

D O I：

10.1016/0167-4838(94)00206-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Distinct alpha- and gamma-type gliadins, as well as a few low molecular weight components have been identified as coeliac immunoreactive proteins from a chloroform/methanol extract from wheat endosperm. Characterization of these components involved the combination of reverse-phase high-performance liquid chromatography, immunoblotting following SDS-PAGE using a coeliac serum and microsequencing analysis. This has allowed the identification of a group of gliadins with different molecular weights, according to their N-terminal amino-acid sequence: five alpha-type gliadins of 31, 35, 38 and two of 45 kDa, one gamma(2)-type gliadin of 40 kDa, two gamma(3)-type gliadins of 31, and 50 kDa, and two gamma-type gliadins with an atypical gliadin N-terminal of 31, and 40 kDa, as well as a few unidentified low molecular weight components and three N-terminal blocked proteins, ah exhibiting similar antigenicity.

引用

页码：143 / 148

页数：6

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