BIOSYNTHESIS OF ISOCITRIC ACID FROM ETHANOL BY YEASTS

被引：32

作者：

FINOGENOVA, TV

SHISHKANOVA, NV

FAUSEK, EA

EREMINA, SS

机构：

[1] Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Pushchino, Moscow Region

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 36卷 / 02期

关键词：

D O I：

10.1007/BF00164426

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The ability of yeasts of different taxonomic groups to synthesize isocitric (ICA) and citric acids (CA) was studied during their growth on ethanol medium. The most active producer of ICA from ethanol, Candida lipolytica 704, was selected. We have chosen cultivation conditions, i.e. pH of the medium and concentrations of oxygen and substrate, optimal for accumulation of ICA. The yield of ICA exceeded 60% of the weight of substrate used. We have studied the dynamics of acid production under conditions of periodic growth of C. lipolytica 704 on ethanol. On the basis of the analysis of citrate and glyoxylate cycle enzyme activity we have developed a conception for the mechanism of ICA overproduction.

引用

页码：231 / 235

页数：5

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