PRIMARY STRUCTURES AND EXPRESSION FROM CDNAS OF RAT OPIOID RECEPTOR DELTA-SUBTYPES AND MU-SUBTYPES

被引：277

作者：

FUKUDA, K ^{[1
]}

KATO, S ^{[1
]}

MORI, K ^{[1
]}

NISHI, M ^{[1
]}

TAKESHIMA, H ^{[1
]}

机构：

[1] INT INST ADV STUDIES,KYOTO 604,JAPAN

来源：

FEBS LETTERS | 1993年 / 327卷 / 03期

关键词：

OPIOID RECEPTOR; CDNA CLONING; CDNA EXPRESSION; RNA BLOT HYBRIDIZATION ANALYSIS; RAT BRAIN;

D O I：

10.1016/0014-5793(93)81011-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequences of rat opioid receptors (designated as ROR-A and ROR-B) have been deduced by cloning and sequencing the cDNAs. The ligand-binding properties of ROR-A and ROR-B expressed from the cloned cDNAs in Chinese hamster ovary cells correspond most closely to those of the pharmacologically defined delta- and mu-opioid receptor subtypes, respectively. RNA blot hybridization analysis revealed that cerebrum and brainstem contain both ROR-A and ROR-B mRNAs, whereas neither ROR-A nor ROR-B mRNAs can be detected in cerebellum.

引用

页码：311 / 314

页数：4

共 22 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].