POSITIVE SELECTION OF RECOMBINANT PLASMIDS BASED ON THE ECOK RESTRICTION ACTIVITY OF ESCHERICHIA-COLI K-12

被引：4

作者：

DEBACKER, O

CHOMEZ, P

DEPLAEN, E

机构：

[1] Ludwig Instituto for Cancer Research, Brussels Branch

来源：

GENE | 1994年 / 150卷 / 01期

关键词：

DNA METHYLATION; DNA MODIFICATION; N-6-METHYLADENINE; RECOMBINANT DNA; CLONING VECTOR;

D O I：

10.1016/0378-1119(94)90885-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a pTZ19R-derived vector which allows efficient positive selection of recombinant plasmids. The system uses the EcoK restriction activity of Escherichia coli K-12. to select against non-recombinant plasmids. The vector contains an EcoK site which, if deleted or disrupted by ligating a DNA fragment, yields recombinant plasmids that are no longer susceptible to EcoK restriction when transformed into a restriction-proficient E. coli host.

引用

页码：197 / 198

页数：2