QUANTITATIVE-DETERMINATION OF AFLATOXIN B-1 IN CHICK LIVER

被引：3

作者：

ESPADA, Y

GUITART, R

ARBOIX, M

机构：

[1] Departments of Veterinary Medicine and Pharmacology and Toxicology, Faculty of Veterinary, Autonomous University of Barcelona, Bellaterra

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1991年 / 8卷 / 02期

关键词：

AFLATOXIN B-1; ONE-STEP EXTRACTION PROCEDURE; RP-HPLC; CHICK LIVER;

D O I：

10.1080/02652039109373966

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method for the rapid and quantitative determination of aflatoxin B1 from small quantities of liver, around 1-2 g, is described. The extraction procedure involves acidification to pH 2 of the aqueous liver homogenates, extraction with chloroform: acetone and HPLC-fluorimetric detection after derivatization with trifluoroacetic acid. Quantitative recovery of aflatoxin B1 from chick liver was achieved and detection at levels of 0.2-1 ppb was proved feasible. The aflatoxin B1 concentration in chick liver after oral administration is also shown.

引用

页码：163 / 170

页数：8

共 19 条

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