MOLECULAR ANALYSIS OF HEM6 (HEM12) IN SACCHAROMYCES-CEREVISIAE, THE GENE FOR UROPORPHYRINOGEN DECARBOXYLASE

被引：8

作者：

DIFLUMERI, C ^{[1
]}

LAROCQUE, R ^{[1
]}

KENG, T ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT MICROBIOL & IMMUNOL,3775 UNIV ST,MONTREAL H3A 2B4,QUEBEC,CANADA

来源：

YEAST | 1993年 / 9卷 / 06期

关键词：

SACCHAROMYCES-CEREVISIAE; HEME BIOSYNTHESIS; UROPORPHYRINOGEN DECARBOXYLASE;

D O I：

10.1002/yea.320090608

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HEM6 (HEM12) in Saccharomyces cerevisiae encodes uroporphyrinogen decarboxylase, the fifth enzyme in the heme biosynthetic pathway. The HEM6 (HEM12) gene was cloned by complementation of heme auxotrophy of a hem6 mutant. Sequence analysis revealed an open reading frame of 1086 nucleotides. The predicted amino acid sequence of HEM6 (HEM12) shows extensive homology to those reported for uroporphyrinogen decarboxylase from mammalian sources. Expression of HEM6 (HEM12) was investigated and was found to increase two-fold in a non-fermentable carbon source. However, HEM6 (HEM12) transcription was unaffected by heme or by intermediates in the heme biosynthetic pathway. In addition, HEM6 (HEM12) expression is not regulated by the transcriptional activator complex HAP2-3-4, as has been shown for some genes encoding heme biosynthetic enzymes.

引用

页码：613 / 623

页数：11

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