RAPID ISOLATION AND PURIFICATION OF LACTOCOCCAL BACTERIOPHAGE DNA WITHOUT THE USE OF CESIUM-CHLORIDE GRADIENTS

Intact bacteriophage of Lactococcus lactis were recovered from small volumes of lysate by centrifugation at 15 000 g without precipitation with polyethylene glycol and sodium chloride, or ultracentrifugation in a caesium chloride gradient. DNA was then extracted and purified by standard protocols. This DNA was readily digested with restriction endonucleases and used successfully in hybridization experiments.