MACROPOROUS HYDROPHOBIC CLOTH (POLYMACRON) AS A SOLID-PHASE FOR NUCLEIC-ACID PROBE HYBRIDIZATIONS

Polyester cloth (polymacron) was employed as a solid phase onto which polymerase chain reaction (PCR) products from the Listeria monocytogenes hlyA gene were dot blotted and sensitively detected by hybridization with nucleic acid probes. This inexpensive solid phase exhibited a high DNA binding capacity and provided ease of handling and washing between each reaction step, resulting in simple hybridization procedures for PCR product detection.