DEVELOPMENT OF A POLARIZATION FLUOROIMMUNOASSAY FOR SULFAMETHAZINE USING AN AUTOMATED ANALYZER

被引：22

作者：

EREMIN, SA

LANDON, J

SMITH, DS

JACKMAN, R

机构：

[1] ST BARTHOLOMEWS HOSP,DEPT CHEM PATHOL,LONDON EC1A 7BE,ENGLAND

[2] CENT VET LAB,BIOCHEM DISCIPLINE,ADDLESTONE KT15 3NB,SURREY,ENGLAND

来源：

ANALYST | 1994年 / 119卷 / 12期

关键词：

ANTIBIOTIC ASSAY; IMMUNOASSAY; FLUORESCENCE POLARIZATION; TISSUE RESIDUE; SULFAMETHAZINE;

D O I：

10.1039/an9941902723

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, non-isotopic, polarization fluoroimmunoassay for the antibiotic sulfamethazine has been developed. The influence of the structure of fluorescein-labelled tracers on the sensitivity of the assay was investigated. Reagents were adapted for use with the Abbott TDx Analyzer and existing software was adapted for automated analyses. Total time for the assay of 1 and 20 samples was 14 and 22 min, respectively. All analytical criteria for the assay were satisfied. The detection limit for sulfamethazine in 20 mu l of aqueous sample was 10 ng ml(-1) (200 pg), which compares well with the sensitivity of enzyme linked immunosorbent assay methods.

引用

页码：2723 / 2726

页数：4

共 7 条

[1] SOLID-PHASE RADIOIMMUNOASSAY OF ESTRADIOL-17BETA [J].