HEAT-TOLERANT METHANOTROPHIC BACTERIA FROM THE HOT-WATER EFFLUENT OF A NATURAL-GAS FIELD

被引:40
作者
BODROSSY, L
MURRELL, JC
DALTON, H
KALMAN, M
PUSKAS, LG
KOVACS, KL
机构
[1] HUNGARIAN ACAD SCI,BIOL RES CTR,INST BIOPHYS,H-6701 SZEGED,HUNGARY
[2] HUNGARIAN ACAD SCI,BIOL RES CTR,INST GENET,H-6701 SZEGED,HUNGARY
[3] HUNGARIAN ACAD SCI,BIOL RES CTR,INST PLANT BIOL,H-6701 SZEGED,HUNGARY
[4] UNIV WARWICK,DEPT BIOL SCI,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND
关键词
D O I
10.1128/AEM.61.10.3549-3555.1995
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Methanotrophic bacteria were isolated from a natural environment potentially favorable to heat-tolerant methanotrophs. An improved colony plate assay was developed and used to identify putative methanotrophic colonies with high confidence. Fourteen new isolates were purified and partially characterized. These new isolates exhibit a DNA sequence homology of up to 97% with the conserved regions in the mmoX and mmoC genes of the soluble methane monooxygenase (MMO)-coding gene duster of Methylococcus capsulatus Bath. The copper regulation of soluble MMO expression in the same isolates, however, differs from that of M. capsulatus Bath, as the new isolates can tolerate up to 0.8 mu M copper without loss of MMO activity while a drastic reduction of MMO activity occurs already at 0.1 mu M copper in M. capsulatus Bath. The isolates can be cultivated and utilized at elevated temperatures, and their copper- and heat-tolerant MMO activity makes these bacteria ideal candidates for future biotechnological use.
引用
收藏
页码:3549 / 3555
页数:7
相关论文
共 37 条
[1]  
[Anonymous], MICROBIOLOGY
[2]  
BODROSSY L, UNPUB
[3]   Optimization of trichloroethylene oxidation by methanotrophs and the use of a colorimetric assay to detect soluble methane monooxygenase activity [J].
Brusseau, Gregory A. ;
Tsien, Hsien-Chyang ;
Hanson, Richard S. ;
Wackett, Lawrence P. .
BIODEGRADATION, 1990, 1 (01) :19-29
[4]   MOLECULAR ANALYSIS OF THE METHANE MONOOXYGENASE (MMO) GENE-CLUSTER OF METHYLOSINUS-TRICHOSPORIUM OB3B [J].
CARDY, DLN ;
LAIDLER, V ;
SALMOND, GPC ;
MURRELL, JC .
MOLECULAR MICROBIOLOGY, 1991, 5 (02) :335-342
[5]   THE METHANE MONOOXYGENASE GENE-CLUSTER OF METHYLOSINUS-TRICHOSPORIUM - CLONING AND SEQUENCING OF THE MMOC GENE [J].
CARDY, DLN ;
LAIDLER, V ;
SALMOND, GPC ;
MURRELL, JC .
ARCHIVES OF MICROBIOLOGY, 1991, 156 (06) :477-483
[6]   BIOGEOCHEMICAL ASPECTS OF ATMOSPHERIC METHANE [J].
Cicerone, R. ;
Oremland, R. .
GLOBAL BIOGEOCHEMICAL CYCLES, 1988, 2 (04) :299-327
[7]  
DALTON H, COMMUNICATION
[8]   PHENOTYPIC CHARACTERIZATION OF COPPER-RESISTANT MUTANTS OF METHYLOSINUS-TRICHOSPORIUM OB3B [J].
FITCH, MW ;
GRAHAM, DW ;
ARNOLD, RG ;
AGARWAL, SK ;
PHELPS, P ;
SPEITEL, GE ;
GEORGIOU, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (09) :2771-2776
[9]  
FOO EL, 1978, PROCESS BIOCHEM, V3, P23
[10]   APPLICATIONS OF A COLORIMETRIC PLATE ASSAY FOR SOLUBLE METHANE MONOOXYGENASE ACTIVITY [J].
GRAHAM, DW ;
KORICH, DG ;
LEBLANC, RP ;
SINCLAIR, NA ;
ARNOLD, RG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) :2231-2236