FACTORS INFLUENCING THE USE OF AQUEOUS 2-PHASE PARTITION FOR PROTEIN-PURIFICATION

Five aspects of ATPP have been selected for review. The first involves the choice of polymers for a two-phase partition. The second concerns the generation of phase potentials and the consequences for protein purification. The third addresses the effect of protein structure on partition behavior. The fourth focuses on the use of affinity partition for bioseparations. And the fifth deals with the problem of separating protein products from phase systems once the protein has been partitioned. All five of these topics have significant implications in the engineering decisions one must make concerning the use of ATPP in protein purification processes.